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Genetic sequencing shows just how similar these species are. Only 12 of their genes differ―less than 0.1% of their genomes.
Intriguingly, one of the 12 is part of one of the sex chromosomes, hinting at a role for sexual selection.
Another three of them play a role in the colouration of male plumage, which is also suggestive.
For, although the species are of similar sizes and shapes, adult male tawny-bellied seedeaters, as their name implies, have brownish-orange chests, while male Ibera seedeaters have black throats and sandy-coloured bodies.
Their songs are distinct, too. They use the same range of frequencies, but different syllables.
First, Ms Turbeck and her colleagues established that females of both species did, indeed, regularly select the right mates.
Observation of pairs suggested so, and genetic testing of young (needed because more than half the females under study mated with extra males in addition to their long-term partners) did not find any exceptions, either.
That done, they looked at males' abilities to distinguish conspecific rivals from heterospecific neutrals.
During the 2019 breeding season they mounted decoy males in the territories of 40 male tawny-bellied seedeaters and 36 male Ibera seedeaters.
They then monitored the residents' responses when a female was around.
They used four decoys. One had the plumage and song of a male Ibera seedeater. One similarly resembled a male tawny-bellied seedeater.
基因测序显示了这些物种的极度相似性。他们只有12个基因不同――不到基因组的0.1%。
有趣的是,这12条染色体中有一条是其中一条性染色体的一部分,这暗示了性选择的作用。
另外三条对雄性羽毛的颜色也有影响,这也很有启发性。
因为,尽管这两个物种的大小和形状相似,但成年雄性茶腹食籽雀(如它们的名字所示)的腹部是茶色的,胸部是橙色的,而雄性伊比利亚食籽雀的喉咙是黑色的,身体是沙色的。
此外它们的鸣啭也截然不同,使用的频率范围是相同的,但音节不同。
首先,图贝克女士和她的同事证实了一点,即这两个物种的雌性确实会定期选择合适的配偶,
对配偶的观察也证实了这一点,而且对年轻配偶进行的基因检测(需要做是因为在研究中有超过一半的雌性除了长期伴侣外还与其他雄性交配)也没有发现任何例外。
研究完成后他们观察了雄性区分同种对手和异种中性对手的能力。
在2019年的繁殖季节,他们在40只雄性茶腹食籽雀和36只雄性伊比利亚食籽雀的领地上安置诱饵雄性。
然后,当有雌性在附近时,他们监测“居民们”的反应。
他们用了四种诱饵。一种有着雄性伊比利亚食籽雀的羽毛和鸣啭,一种和雄性茶腹食籽雀相似。
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